Alternative product Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. An internal reference dye, such as ROX, corrects well-to-well optical variations, and is used for fluorescent signal normalization. SYBR Green I dye is ideal for target identification (screening assays) or when a limited number of assays is needed. Many PCR machines require a passive reference dye. SYBR ® Green master mixes are available with other dyes including ROX. SYBR ® Green master mixes are 2x concentrated, and contain dNTPs, MgCl 2, and DNA polymerase.
This minimizes well-to-well variability that can result from a variety of causes, such as pipetting error or sample evaporation. Passive Internal Reference 1 is provided to normalize non-PCR–related fluorescence fluctuations. The hot-start enzyme AmpliTaq Gold® DNA Polymerase minimizes nonspecific product formation (including primer-dimers), yielding superior performance and sensitivity. SYBR® Green I dye is a double-stranded DNA binding dye that detects any double-stranded DNA generated during PCR. Proprietary buffer enhancements ensure performance and reliabilityĪpplied Biosystems® SYBR® Green PCR Master Mix provides maximum flexibility at a reduced cost because no target-specific TaqMan® probes are required.dUTP significantly reduces carryover contamination when used in conjunction with uracil-DNA glycosylase.AmpliTaq Gold® DNA polymerase minimizes nonspecific product formation to achieve superior performance.SYBR® Green I dye detects double-stranded DNA, so specific probes are not required.Premixed components stored at 2–8☌ significantly reduce assay setup time.Applied Biosystems® SYBR® Green PCR Master Mix combines SYBR® Green I dye, AmpliTaq Gold® DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial. Everything you need for SYBR® Green dye-based PCR amplification and detection in a convenient, single-tube format.